Antibiotic Sensitivity Lab – 1 Page A. Write a lab report about the Antibiotic Sensitivity−Kirby-Bauer Diffusion Test experiment in which you do the follo

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Antibiotic Sensitivity Lab – 1 Page

A.  Write a lab report about the Antibiotic Sensitivity−Kirby-Bauer Diffusion Test experiment in which you do the following: 

1.  Explain why selective toxicity is an important feature of antibiotics. 

a.  Discuss the relative benefits of broad-spectrum and narrow-spectrum antibiotics.
 

Note: The explanation from part A1 and discussion from part A1a belong in the introduction section of the lab report.
 

2.  Describe the steps you followed to complete the Kirby-Bauer diffusion test.

Note: Include any changes from the normal protocol that might have been incorporated into your experiment.

Note: The description from part A2 belongs in the materials and methods section of the lab report.
 

3.  Record the observed results of the Kirby-Bauer diffusion test, including the zone of inhibition measurement and interpretation (e.g., resistant, intermediate, susceptible) for each antibiotic using the attached “Data Table.”

Note: The explanation from part A3 belongs in the results section of the lab report.
 

4.  Based on your recorded results from the Kirby-Bauer diffusion test, explain whether each of the following antibiotics would be a reasonable choice for treating S. epidermidis: 

•  penicillin 

•  novobiocin 

•  gentamicin
 

Note: The explanation from part A4 belongs in the discussion section of the lab report.

Antibiotic Lab website https://web5.wgu.edu/aap/content/antibiotic_sensitivity-kirby_bauer–diffusion_test.pdf

Antibiotic Susceptibility Zone: Diameter Interpretation

Zone Diameter Standards (mm)

Antibiotic Name

Antibiotic

Code

Resistant

Intermediate

Susceptible

Penicillin

P-10

<28

28

28

>29

Gentamicin

GM-10

<12

13-14

>15

Novobiocin

NB-30

<17

18-21

>22

Microbiology Task 3 Tables

Task 3 Data Table 1

Staphylococcus epidermidis

Zone of inhibition measurement

Interpretation

Penicillin

 

 

Gentamicin

 

 

Novobiocin


 

 

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